The applications of viral protein cages have expanded rapidly into the fields of bionanotechnology and materials science. However, the low-cost produc…

The positively transformed yeast cells were cultured for about 20 h in a shaking flask comprising 50ml Buffered Glycerol Complex Medium (BMGY, 1% yeast extract,2% peptone, 100mM potassium phosphate buffer, pH 5.0, 1.34% YNB, 4 × 10-5 % biotin, and 1% glycerol) to OD 600 = 4.0. BMGY medium (buffered glycerol complex medium) contained 10 g/liter Bacto yeast extract, 20 g/liter hipolypepton, 13.4 g/liter yeast nitrogen base without amino acids (YNB) (BD Biosciences), 0.4 mg/liter biotin (Nacalai Tesque), 100 mM potassium phosphate buffer (pH 6.0), and 20 g/liter glycerol. Oct 15, 2018 · The protein expressing strains were obtained using G418 selection and cultured in 25 ml of buffered glycerol-complex medium [BMGY; 1% yeast extract, 2% peptone, 100 mM potassium phosphate (pH 6.0 Oct 29, 2010 · Mut S phenotype integrated into the grown colonies with the AOX1 locus of the yeast genome were selected and grown in 1 L of buffered glycerol complex medium to an optical density over 6 at 600 nm. Cells were induced with 1% methanol by replacing the buffered glycerol complex medium with buffered methanol complex medium. Apr 29, 2003 · Protein expression was carried out at room temperature in a 96-well-plate format with buffered glycerol-complex medium (BMGY) consisting of 1% yeast extract, 2% peptone, 100 mM potassium phosphate buffer (pH 6.0), 1.34% yeast nitrogen base, 4 × 10 −5 % biotin, and 1% glycerol as a growth medium. The induction medium was buffered methanol

BMGY medium (buffered glycerol-complex medium) was prepared according to the manual of the EasySelect Pichia Expression Kit (Invitrogen, USA): 1% yeast extract, 2% peptone, 100 mM potassium phosphate, pH 6.0, 1.34 % YNB, 4×10-5% biotin, and 2% glycerol. Expression of the recombinant Laccase

Oct 02, 2012 · The P. pastoris transformants were cultured in 25 mL of buffered glycerol-complex medium (BMGY) shaken at 28°C and 250 rpm in 250-mL glass flasks. When cultures reached an OD 600 of about 6, the cells were centrifuged and resuspended in 100 mL of buffered methanol-complex medium (BMMY) to an OD 600 of 1.0, shaken at 28°C and 250 rpm in 500-mL After choosing the most resistant colon, a single colony of multiple inserted His −, Mut + GS115 recombinants was inoculated into 25 mL buffered glycerol complex medium (BMGY; 1% yeast extract, 2% peptone, 100 mM potassium phosphate (pH 6.0), 1.34% yeast nitrogen base (YNB), 4 × 10 - 5% biotin, 1% glycerol) and cultured at 250 rpm and 30°C

Twenty transformants were used to inoculate 40 ml of buffered glycerol complex medium (BMGY) (Invitrogen) (0.1 M potassium phosphate buffer at pH 6.0 containing 1% [wt/vol] yeast extract, 2% [wt/vol] peptone, 1.34% [wt/vol] yeast nitrogen base without amino acids, 1% [vol/vol] glycerol, and 4 × 10 −5 % [wt/vol] biotin). Cultures were grown

The transformants were grown on minimal dextrose (MD) agar plates at 32°C for 48 h. Ninety-six colonies were randomly selected to grow in 2-mL buffered glycerol complex medium (BMGY) at 30°C for 48 h. The cells were collected by centrifugation (12,000g) and resuspended in 2-mL buffered methanol complex medium (BMMY). After 48-h induction with Buffered Glycerol Complex Medium, Sterile 2.0% Peptone, 1.0% Yeast extract, 1.34% Potassiumphosphate pH 6.0, 1.34% Yeast nitrogen base w/o AA, 0.4 mg/L Biotin, 1.0% Feb 27, 2017 · Buffered glycerol-complex medium. CD: Circular dichroism. HPLC: High performance liquid chromatography. pNPR: p-Nitrophenyl α-L-rhamnopyranoside. YNB: Yeast nitrogen base. YPD: Yeast extract peptone dextrose medium Applications of the GST- Affinity Tag in the Purification and Characterization of Proteins A dissertation submitted in partial fulfillment of the requirements for the degree of